FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23463768
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT273330.1164902414650537Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG31300.029.5291941
TAGGACG83400.023.1715374
TTGACGG40850.021.74255831
TCTCGGG66200.021.659275120
CGTATCC218000.019.951958120
CGCCGTT48550.019.400566120
GGACGTG101150.018.212796
CGGTGGG89750.017.44657120
GTCCTAC152500.016.7260861
TCGCCGG123650.016.690462120
CCGACGG67750.016.647635120
CGTCTTA33150.016.10924715
CTACGGT28200.015.53088713
AGGACGT143550.015.3851025
CGGCTAT62600.015.33833735
TACAGTG174250.015.1542945
GTATAGT142850.014.9150021
ATTCGAA35950.014.85703332
GACGGCT57800.014.84735933
AACTGCG50200.014.8208427
Produced by FastQC (version 0.11.2)