FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23463768
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA261460.11143137794407106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG31450.033.9518281
CTAGACG32500.023.810543
CTACGGT30200.023.6370713
TACGGTG31700.022.89750114
TTGACGG43100.022.68486631
TAGGACG86150.021.7947524
TAGACGC66700.020.2357624
TCCGCAA37600.019.30293733
CCTACGG37800.018.88444112
GGACGTG99800.018.5713186
GTCCGCA40500.018.21696332
CGGCTAT62500.018.13876335
GTCCTAC154550.018.0487771
ACGCACC42800.017.6579257
TTAGACG37050.017.648233
GACGCAC44400.017.4269566
GACGGCT56500.017.41090233
GTAGGAC158750.017.382293
CCTATAC97250.016.1612363
TGACGGC63000.016.09059132
Produced by FastQC (version 0.11.2)