FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23508069
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT272340.11584958339198341Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32400.024.634171
TCTCGGG66700.023.29665120
CGTATCC222300.019.78263120
CGCCGTT49200.019.754656120
TAGGACG85100.019.7451214
CGGTGGG86200.018.792131120
TTGACGG41000.018.7331331
CTACGGT28450.018.13602813
GTCCTAC157700.017.276491
TCGCCGG126050.017.134806120
TACGGTG30250.016.26403614
GGACGTG99800.016.053056
TAGACGC70700.015.8727994
CGTCTAA34250.015.5941151
CGTAATC37600.015.47897522
GGTACGA35300.015.4675118
ATTCGAA37650.015.45930832
CTAGACG35400.015.4265733
CGTGATT66850.015.25916628
TACAGTG185600.015.22915
Produced by FastQC (version 0.11.2)