FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23508069
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA260930.11099593080146225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG31550.036.7001761
TTGACGG45250.024.52540631
TAGGACG91800.020.3231454
GGTACGA35050.020.1949718
CTAGACG33700.019.225033
GTCCTAC151450.018.8955381
CGGCTAT60600.018.80822235
GACGGCT56950.018.74948333
TACGGTG32150.018.472314
GTACGAG39050.018.27995519
TACGAGG38850.018.2198120
TACACGG42700.018.123135
TAGACGC64850.017.9458894
CTACGGT32350.017.8017913
ACGGCTA59300.017.39950234
GGACGTG107600.017.0587526
CTACACG43650.017.041614
CGTACGT33800.017.0379634
GTAGGAC160150.016.968543
TGACGGC65750.016.69622832
Produced by FastQC (version 0.11.2)