FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23552183
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT268140.11384931919049712Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT27700.059.4015294
CGACGTA50950.036.96558492
TGTCGGT43750.036.67717793
ACGTATC47800.036.5567294
TACGAGT26000.036.3499294
CACCGTA46850.035.70158893
TAGATCG39400.034.8602888
GTGGACG56200.030.36689890
CTCGTAT25550.030.07111591
TCGGTAG32800.029.64342389
CGCAGTA55300.029.50856491
ACGCCGT49650.029.44294294
TGGACGC57800.028.9380591
GGTCGAC67000.028.71956389
TCGACGT65900.028.68278991
TAGTCGC32900.028.10638693
TGGTCGA68400.027.93353588
ATGGTCG35000.027.58558894
AGTGTCG43800.027.32151289
CTACGAG35000.027.1970693
Produced by FastQC (version 0.11.2)