FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23552183
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA255370.10842731648272265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG31600.031.5160061
CTAGACG33400.022.0936243
CTACGGT31550.021.67607313
TTGACGG43500.021.37574631
TACGGTG32000.021.183214
TAGGACG87750.020.5797734
TAGACGC68000.019.4104214
TCCGCAA39450.017.79170833
GTCCTAC150650.017.6418761
TTATCGT39000.017.38204850
CCTACGG40450.017.35171712
TACGCGT51450.016.6752325
GACGCAC45400.016.6489936
ATACGCG51750.016.5785644
GGACGTG103850.016.5208666
ACGCGTA53150.016.2529626
GTCCGCA42600.016.1944832
TTAGACG37850.016.0089863
GACGGCT54750.015.99733233
GTAGGAC157250.015.9857023
Produced by FastQC (version 0.11.2)