FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23558102
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG52100.030.74898120
CGTCTAG34200.030.5312061
CGCCGTT30650.027.01505120
CGGTGGG62100.024.638016120
TTGACGG42200.024.17161231
CCGACGG42750.022.877506120
TCGCCGG76150.022.849949120
CGTATCC154900.021.420563120
TGACGGC50250.020.5381632
GACGGCT48150.020.43689333
TAGGACG68550.019.2591024
TAGGGCG46550.018.81925231
GGGCGTT46450.018.60133433
TACGGTG26450.018.59996814
CTACGGT27000.018.44328713
CGTACGT35950.017.85864634
TCAACGG35000.017.65603413
TCCGCAA27650.017.57750333
ACGGACC36800.017.28159716
GGACGTG75100.017.2563486
Produced by FastQC (version 0.11.2)