FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23558102
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT320120.1358853102851834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28250.034.612171
TTGACGG39250.023.22953831
CTACGGT25800.020.45954113
TGACGGC45950.020.23404932
TACGGTG26950.019.586514
GGTACGA34950.019.39542218
GACGGCT47250.019.29649433
TAGGACG72100.019.2208794
CGTACGT36900.018.6945734
TACGAGG38750.018.42227420
TTGGACG41700.018.26999317
TCCGCAA28500.017.89001533
GTACGAG38550.017.58421319
TCTTACG42000.017.56671744
TGGACGT42700.017.56103518
GCGAATT40100.017.05412319
CCGTACG40150.016.8822233
GATGCGA39800.016.8810416
CGAATTG39600.016.8142822
AACTGCG45050.016.7781377
Produced by FastQC (version 0.11.2)