FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23509775
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32450.031.6221791
CGCCGTT31450.025.565313120
TCTCGGG53150.025.174946120
CGGTGGG65950.025.01986120
TCGCCGG77000.023.767094120
TTGACGG40650.023.17520731
CCGACGG40600.021.724922120
CTACGGT26150.020.64899813
TGACGGC47700.020.25312232
GACGGCT46500.020.00148833
CGTATCC157850.019.880352120
TAGGACG73100.018.962914
TACGGTG28250.018.9016514
GGTACGA35250.017.87146618
TCCGCAA29900.017.86086333
TGACGCC52900.017.807829120
CGTACGT37950.017.23441134
GCGAATT39350.017.07696319
GTCCGCA31750.017.00917832
CCTACGG31500.016.9514812
Produced by FastQC (version 0.11.2)