FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23509775
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT319220.13578181841383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32150.037.3190841
TTGACGG41350.027.1283331
GACGGCT45800.023.5756733
TGACGGC49300.023.24039332
TACGGTG28400.022.60010514
CTACGGT28600.022.44206213
TAGGACG71450.020.9926744
GGTACGA35450.020.13795718
TCCGCAA32500.019.7495633
CTAGACG36050.019.6384283
CTGGTCG44450.019.5697639
TGGTCGG41800.019.51675610
CCTACGG33100.019.39102612
GACGCAC35800.018.936256
GTCCGCA34900.018.56330532
ACGGTGA37550.018.37166815
GTACGAG38950.018.32912619
TAGACGC73450.018.2155824
TACGAGG39950.018.17078220
CGGCTAT70000.018.0820635
Produced by FastQC (version 0.11.2)