FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23476752
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32600.034.4211461
TTGACGG37800.028.2615631
TCTCGGG51400.026.614796120
TCGCCGG72600.025.537197120
CGGTGGG63500.024.377958120
TGACGGC45800.023.45608332
CGTATCC158900.023.10888120
GACGGCT44000.022.50523233
CGCCGTT27550.021.125233120
CTACGGT26600.020.75073613
CCGACGG39750.020.377365120
TACGGTG27300.019.5593614
GGTCGGT69000.018.69462211
CGGCTAT64500.018.51159335
TCCGCAA28700.017.98327433
CGTCTTA35800.017.76438515
AGACCGT46350.017.7344826
TAGGACG68950.017.4929524
GGTACGA32750.017.40365818
TGACGCC53000.017.32076120
Produced by FastQC (version 0.11.2)