FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23476752
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT314800.13409009900517754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30650.036.013771
TTGACGG41850.024.51097931
CTACGGT26650.022.73361213
TACGGTG27600.021.73382414
GACGGCT48150.020.68101133
TGACGGC50550.020.64848132
TAGGACG72850.020.341284
TCCGCAA30250.020.02884933
GACGCAC32150.018.8456656
CCTACGG31350.018.7513612
GTCCGCA31400.018.7221832
GGACGTG81600.018.1584156
GTAGCGT38900.017.8907124
CGTCGTG39100.017.79605328
TAGACGC70600.017.5904334
AGCGTCG40500.017.18157426
GCGTCGT40550.017.15969527
GGGCGTT42850.017.0792633
GGTACGA37400.017.002518
CGACGAA36800.016.95613923
Produced by FastQC (version 0.11.2)