FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23473561
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32600.029.8193741
TCTCGGG51200.028.240614120
TCGCCGG70050.025.009315120
CGGTGGG66350.024.866741120
TTGACGG37850.023.30687331
CTACGGT25550.021.83901213
CCGACGG39700.021.76201120
CGCCGTT27000.021.554356120
CGTATCC160750.021.386057120
TGACGGC44000.020.59476532
TACGGTG27750.020.32384314
GACGGCT42850.020.02708633
TCCGCAA27750.018.81433133
CCTACGG31800.018.11277812
TAGGACG71900.018.0265664
GTCCGCA31150.017.91667632
GGGCGTT45300.017.88414233
GCGAATT39850.017.46619819
CGAATTG42700.017.14354522
TGACGCC53600.017.125914120
Produced by FastQC (version 0.11.2)