FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23473561
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT314500.13398052387535064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG33200.036.5026321
TCGTACG4600.029.99612697
TTGACGG39100.028.68803631
CTACGGT28400.027.24733413
TACGGTG29150.026.34089314
TGACGGC45900.024.43795632
GACGGCT45100.024.0733833
TCCGCAA32050.023.58183333
GTCCGCA33150.023.16127232
CTAGACG36350.022.2816183
CCTACGG34800.022.06404912
GACGCAC37600.021.8585996
ACGGTGA38050.020.810315
ACGCACC39300.020.1490847
TAGACGC72900.019.8338434
GGTACGA34400.018.83325218
TGGTCGG38800.018.7077310
CGGCTAT65250.018.66167335
CTGGTCG43200.017.9132849
ACGGCTA62000.017.70493934
Produced by FastQC (version 0.11.2)