FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23405945
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG35950.030.5498181
TCTCGGG51950.028.98677120
CGCCGTT29050.028.706505120
TTGACGG40600.024.83354831
CGGTGGG64550.024.16511120
TCGCCGG77100.023.966686120
GACGGCT44900.021.65330533
CCGACGG36600.021.637373120
CTACGGT26350.021.40309313
TGACGGC47050.021.30160532
TACGGTG27200.020.95567314
CGTATCC159750.020.655394120
TCCGCAA28150.020.04033333
TGACGCC57900.018.962004120
GGTACGA33550.018.77787618
GTCCGCA31750.018.71315232
CGTACGT35700.018.65958234
CCTACGG31750.018.32978212
CTAGACG33400.017.9684243
CCGTACG36900.017.89047233
Produced by FastQC (version 0.11.2)