FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23405945
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT312330.13344045711463476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29900.034.7118531
TTGACGG40450.024.46848331
CTACGGT29750.024.19531613
TCCGCAA28900.024.07686433
TACGGTG29800.023.55321714
GACGGCT45650.022.07538633
CGTACGA4650.021.93163580
GTCCGCA31750.021.91563432
TAGGACG71150.021.332654
TGACGGC47300.021.17849732
GGTACGA35000.020.91101618
CTAGACG38350.019.7109743
GACGCAC36800.019.3997236
CCTACGG35900.019.38320512
ACGGTGA36250.019.36236815
GTACGAG39150.018.54219419
TACGAGG40300.018.31073220
GGACGTG80200.018.1772546
AACTGCG47100.017.8306567
ACGCACC39650.017.7012067
Produced by FastQC (version 0.11.2)