FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23389694
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG33650.028.537651
TCTCGGG49950.026.783937120
CGCCGTT27900.024.51352120
TCGCCGG70050.024.494179120
CGGTGGG58500.024.40766120
TTGACGG40550.022.3476731
CCGACGG39900.021.802189120
CTACGGT25950.020.11471213
TACGGTG27450.019.4538114
TAGGACG70800.018.9884174
GGTACGA34500.018.78305818
CGTATCC170750.018.762278120
TGACGGC48300.018.63761132
CGTCTTA37050.018.4619415
GACGGCT45650.018.404933
GTACGAG36000.018.16737419
TGCTCGA42550.018.0478210
GCTCGAA39150.017.92985311
TACGAGG38100.017.63848120
TAGACCG36450.017.6181474
Produced by FastQC (version 0.11.2)