FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23389694
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT313480.13402484017105995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32450.035.1251341
TTGACGG41150.026.38387731
TGACGGC47950.023.0175532
GACGGCT46550.022.03465733
GGTACGA34950.021.79685618
TAGGACG66850.019.7424584
GTACGAG39550.019.71686619
CTACGGT29750.019.35721813
TACGGTG29900.019.26035314
TACGAGG40800.019.11242720
TCCGCAA31400.018.33882133
CGGCTAT67200.018.20915635
TGCGACC41950.018.16022110
GTCCGCA33750.017.59508132
GACGCAC36250.017.5396616
AACTGCG47250.017.5185387
ACGTACG38550.017.42729637
CGTACGG37600.017.38864338
CTGCGAC47250.017.2651629
GCGAATT37950.017.22882819
Produced by FastQC (version 0.11.2)