FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23440060
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32150.031.9193631
TCTCGGG53650.029.074675120
CGCCGTT32000.025.310165120
TTGACGG39400.024.36796831
TCGCCGG75600.023.093111120
CGGTGGG63200.022.497927120
CCGACGG40450.021.951006120
CGTATCC177450.021.604095120
CTACGGT26150.021.33714913
TACGGTG27000.021.11051614
GACGGCT43700.020.59706733
TGACGGC48200.020.0435432
TCCGCAA30200.019.27348733
GTCCGCA30750.018.53847532
TGACGCC64300.018.474192120
GACCGTT44550.018.4510527
TAGGACG69000.018.438514
CGTCTTA36850.018.2359315
AGACCGT45800.018.0800236
CCTACGG31600.018.03631412
Produced by FastQC (version 0.11.2)