FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23440060
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT314740.1342744003215009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32200.037.0767861
TTGACGG42800.026.63016931
GACGGCT45900.023.65538233
TGACGGC49350.023.09566732
CTACGGT26050.019.80408713
TACGGTG26450.019.50459314
TGCGACC41850.019.06370510
ATTCGAA37550.018.69132232
CGGCTAT68100.018.67482635
AACTGCG47200.018.554297
ATCATCG43850.018.33065630
CATCGTT43500.018.20324332
GCGACCA45350.018.1217811
GCGAATT41100.017.95225519
TTGGACG40450.017.94436817
CGAATTG38800.017.934222
CGTACGT37100.017.62449634
CGACCAA44800.017.5408612
CCGTACG38400.017.34027133
TAGGACG72200.017.2822914
Produced by FastQC (version 0.11.2)