FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23510761
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT26700.068.84122594
CGACGTA39000.052.89006492
TCGACGT41100.050.35365791
TGTCGGT30300.049.65302793
ACGCCGT28900.049.46722894
ACGTATC41500.046.9152594
GGTCGAC44450.046.4064789
TGGTCGA48650.042.6814888
GTCGACG49050.042.19246390
GTCACCG46650.042.02813391
CACCGTA44000.041.6193993
GTCGGTG36450.041.08859394
TACGAGT21750.041.00229394
GTGATCG24250.040.42499591
TAGATCG32900.040.1447188
TGGACGC41500.038.54952291
GTGGACG41650.038.24733490
GTCGATC30550.037.43640592
GTAGCCG51450.037.3129792
TGATCGC27050.037.24693792
Produced by FastQC (version 0.11.2)