FastQCFastQC Report
Mon 11 Dec 2017
AJ-M-ATAC-2-2_CGTACTAG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-M-ATAC-2-2_CGTACTAG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23510761
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT307340.1307231186604296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30250.033.319681
TTGACGG38300.024.74715831
CTACGGT28700.024.6641513
TACGGTG29800.023.75332614
TCGTACG4350.023.44871599
TCCGCAA30800.022.78778633
GTCCGCA32100.021.67808332
TAGGACG67400.021.363754
CTAGACG35700.021.3424853
TGACGGC44450.021.18828632
CCTACGG33600.021.06725312
GACGCAC36200.020.8803256
GGTACGA36950.020.45693818
GACGGCT43050.020.06579433
ACGGTGA37850.019.49506815
ACGCACC37150.018.7318237
TCTTACG41050.018.7077744
TACGAGG41500.018.21403120
GGACGTG76700.018.1454586
TGCGACC40750.018.10741810
Produced by FastQC (version 0.11.2)