FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21169252
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT224760.10617285863477839Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30350.034.2059251
TTGACGG35500.027.38125831
TCTCGGG49400.025.14224120
CGCCGTT30400.024.671583120
TCGCCGG72100.024.466545120
GACGGCT41800.023.11070833
TACGGTG26900.022.97268114
CTACGGT25950.022.65767913
CGGTGGG63500.022.205202120
CGTATCC138350.021.901447120
CCGACGG41100.021.022348120
TGACGGC46600.020.98782232
CCTACGG30400.019.34101312
CTAGACG32200.019.1953163
TAGGACG65900.018.8494594
ATTCGAA34300.018.71782532
CGAATTG34150.018.27181622
CCGTACG34200.018.24613633
TCCGCAA31650.018.19957233
CGGCTAT59600.018.12085235
Produced by FastQC (version 0.11.2)