FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21169252
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27350.034.216381
TACGGTG27800.026.5399714
CTACGGT28200.026.37622813
TTGACGG37150.023.7361331
CTAGACG33550.022.8884853
CCTACGG34550.021.7022712
TCCGCAA33800.021.65182533
GACGGCT43150.020.01857233
GACGCAC36650.019.9696336
ACGCACC38300.019.7354837
ACGGTGA39250.019.5625215
TGACGGC45850.019.3630532
GTCCGCA37350.019.27267632
TAGGACG64750.019.1790964
GGTACGA31150.018.68019918
CGTACGT31050.018.54659334
CCGTACG31800.018.48631733
TAGACGC69150.018.1322484
CCGTAAC37050.017.80933429
GGGCGTT40900.017.59986533
Produced by FastQC (version 0.11.2)