FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21142707
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT227500.10760211547177947Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30350.032.6228071
TCTCGGG50350.026.932032120
TTGACGG36700.024.52517131
CGCCGTT29850.024.322107120
TCGCCGG72200.023.518488120
CGTATCC141150.022.359652120
CGGTGGG59150.022.316603120
CTACGGT27450.021.63825413
TACGGTG27850.021.1120414
CCGACGG39800.020.352177120
GACGGCT44200.019.95633933
TGACGGC47000.019.78885732
GCGAATT33600.019.10640519
CCTACGG32350.018.9171312
TCCGCAA30650.018.59854333
CTAGACG33600.018.3947563
TGACGCC53500.018.168596120
GGTACGA31650.017.4399618
GATGCGA36500.017.42377916
CGGCTAT61150.016.97435835
Produced by FastQC (version 0.11.2)