FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21142707
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA212380.10045071333580889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29150.034.7795031
TTGACGG39600.023.63105431
TAGGACG67300.021.3063684
GACGGCT45100.020.48320233
CTACGGT27250.020.03172113
GGTACGA33150.019.90625418
TGACGGC49150.019.40560732
TACGGTG29200.019.10484514
TCCGCAA28850.018.92122533
GTACGAG35950.018.35687619
CTAGACG32550.018.2478123
TTAGACG37300.018.1759133
TAGACGC66750.018.0663914
TACGAGG37900.017.88757320
GGACGTG77400.017.594866
CGGCTAT63500.017.57114635
TCTTACG39950.017.26852444
GGGCGTT39450.017.18245133
CCTACGG32250.017.11202812
ACGTACG35200.017.04154437
Produced by FastQC (version 0.11.2)