FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21173843
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT223680.10563977450857645Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32550.028.3902231
TCTCGGG49550.026.39774120
TTGACGG36600.024.4326731
CGCCGTT28300.023.3217120
CGTATCC145500.023.216637120
CCGACGG40000.022.350136120
GGTACGA32000.022.31142618
TCGCCGG72100.021.719967120
CGGTGGG63500.021.259974120
CTACGGT25750.020.96981413
TACGGTG27200.020.2930914
GTACGAG34300.020.29128619
GACGGCT42200.020.1941833
TGACGGC46750.019.51316532
TACGAGG36800.019.23888620
CTAGACG31400.018.7281323
TCCGCAA29800.018.12493933
CCTACGG31900.017.86743512
TAGGACG66600.017.3892614
GACCGTT38850.017.2969537
Produced by FastQC (version 0.11.2)