FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21173843
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28450.036.4792061
CTACGGT27150.028.05927813
TACGGTG28800.026.6600614
TTGACGG39650.024.20619831
CTAGACG33750.023.2867493
CCTACGG33900.023.1800412
TCCGCAA32200.022.54131533
GTCCGCA34500.021.7340532
ACGCACC36150.021.0750087
GACGGCT44850.020.59719533
GACGCAC37450.020.3433866
TGACGGC47500.020.3320932
TAGGACG68250.019.8663044
ACGGTGA40900.019.50621415
GGACGTG75200.018.427466
AACTGCG41850.018.0612347
AGACCGT43050.017.836446
TAGACGC66700.017.8094564
GACCGTT38850.017.6029787
CGGCTAT59400.017.37029535
Produced by FastQC (version 0.11.2)