FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21093973
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT223030.10573162296168674Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29150.029.231781
TCTCGGG48000.025.248423120
CTACGGT26050.024.8745713
TACGGTG26600.024.58580414
TCGCCGG73050.023.571423120
CCGACGG39100.023.016468120
CGCCGTT28250.022.93662120
TTGACGG35100.022.22715631
CGTATCC137450.021.737457120
TCCGCAA30300.021.58895333
CGGTGGG59450.021.394796120
CCTACGG31500.020.76134712
CTAGACG33750.020.2691973
GTCCGCA33750.019.20426432
TAGGACG60600.019.1107544
TGACGCC53400.018.762873120
TAGACGC67050.018.5252764
GACGGCT44200.018.05830833
ACGGTGA37750.017.80084615
GACGCAC36950.017.7010486
Produced by FastQC (version 0.11.2)