FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21093973
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29050.033.042461
TTGACGG39100.023.62520631
CTACGGT26600.023.45352413
TACGGTG27400.022.33146714
CTAGACG31250.021.6936763
GGTACGA31450.020.0279918
GACGGCT44800.019.9499133
CCTACGG32350.019.84119812
TGACGGC48450.019.43741832
TAGGACG63950.019.1379054
GTACGAG34350.018.86135719
TCGTACG4951.6370905E-1118.1794497
GACGCAC36050.017.9720466
TCCGCAA33350.017.80623233
CGTACGT30950.017.6374210
CCGTACG32600.017.2969159
TACGAGG37300.017.2088520
TAGACGC64200.017.194424
CGGCTAT60650.017.1099235
ACGCACC37150.016.9547337
Produced by FastQC (version 0.11.2)