FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21065798
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT219620.10425429884023384Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG31100.030.4898821
CGCCGTT27500.029.01505120
TCTCGGG53150.026.864454120
TTGACGG36650.023.25314331
CCGACGG39750.022.48814120
CGTATCC146200.022.364206120
CGGTGGG60400.022.149927120
TCGCCGG74400.020.562296120
GACGGCT42950.019.56285333
CTACGGT25150.019.56162813
TGACGGC44600.019.24280732
CGTCTTA31500.018.85689715
TACGGTG26900.018.51272614
TAATCGA29200.018.28869221
GCGAATT37350.016.86841219
AACTGCG45200.016.7267977
CTAGACG31250.016.7081473
CGGCTAT60050.016.68904935
TCCGCAA29800.016.51447933
TAGGACG64750.016.4053424
Produced by FastQC (version 0.11.2)