FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21065798
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27200.034.850671
CTACGGT27650.024.9481913
TACGGTG28150.024.5072714
TTGACGG38800.022.26209631
TCCGCAA30850.022.16588233
CCTACGG33550.021.09915212
GTCCGCA33250.020.7463432
CTAGACG34350.020.2606873
TAGGACG66700.019.608734
GACGGCT43150.019.32276733
GACGCAC37600.019.1476886
TAGACGC69600.018.79174
TGACGGC46500.018.70468332
GGTACGA31800.018.6759918
ACGCACC37650.018.6421227
CCGTAAC36950.018.1818429
TCGCGTA5950.018.14684583
CGAATTG35450.018.10664222
AACTGCG46500.017.5452847
ATTCGAA35000.017.48104732
Produced by FastQC (version 0.11.2)