FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21050116
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT222980.10592815735552241Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30450.030.5501651
TCTCGGG45850.026.431469120
CGCCGTT31000.023.61062120
TTGACGG37300.023.00679631
CGTATCC156250.022.423431120
CGGTGGG64850.021.740381120
TCGCCGG70300.021.591085120
CCGACGG39350.021.497286120
CTACGGT26400.019.9993513
TACGGTG26300.019.62001814
GACGGCT44150.019.43722533
CGTCTTA31200.019.23110415
TGACGGC46850.018.31704532
TCCGCAA27700.018.198233
GGGCGTT40900.018.19396433
ATTCGAA38050.017.82184832
TAGGACG64150.017.7754
TGACGCC58050.017.672709120
CGGCTAT59650.017.30368835
CGAATTG37850.016.96242722
Produced by FastQC (version 0.11.2)