FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21050116
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28250.035.0381
CTACGGT28450.026.56699813
TTGACGG36050.025.45761331
TACGGTG30600.024.50473414
CTAGACG33400.023.528573
CCTACGG33750.022.92777312
TCCGCAA33100.022.47114433
TGACGGC43550.021.21114332
GACGCAC36800.020.7001726
TAGGACG68850.020.1269384
ACGCACC38050.020.0199997
GACGGCT41250.019.77636533
GTCCGCA38200.019.62809632
CCGTACG32250.019.52949733
TAGACGC67550.019.3597974
CGTACGT31700.019.30071834
CTGGTCG44550.019.2539029
ACGGTGA40850.018.79624415
TTGGACG38650.018.3135417
TAGACCG32800.018.1063544
Produced by FastQC (version 0.11.2)