FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21018967
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT218770.10408218443846455Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30200.029.8073441
CGCCGTT31250.027.837214120
TCTCGGG48700.027.102009120
TTGACGG35400.024.4096231
CGTATCC161250.022.28614120
CGGTGGG60300.021.092415120
CTACGGT25900.021.07921813
GACGGCT41300.020.92253333
TCGCCGG70600.019.969671120
TACGGTG27450.019.88975714
CCGACGG41000.019.607794120
TGACGGC44850.019.53404432
TAGGACG67500.018.848254
CGTCTTA31600.018.79773115
TAGGGCG40850.018.6557831
TCCGCAA30850.017.89511333
CTAGACG33800.017.5775153
GGGCGTT42700.017.56644633
TGACGCC60700.017.49413120
TGCGACC40200.017.46085410
Produced by FastQC (version 0.11.2)