FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21018967
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28050.032.936071
TTGACGG37800.023.3284431
CTACGGT27000.023.1064313
TACGGTG30200.021.6512414
TAGGACG66250.020.9164684
CTAGACG32850.020.4524293
GACGGCT42250.020.44541433
TGACGGC46200.019.86596332
CCTACGG32050.019.83973712
TAGACGC63500.018.9882344
TCCGCAA33600.018.74606733
GGTACGA30550.018.6536718
GGACGTG74500.018.357436
GACGCAC35650.018.1717826
CGTCTAA35350.018.158431
GTCCGCA34600.018.030932
ACGCACC37100.017.6232437
CGTACGT29300.016.99302334
CCGTACG30500.016.91448433
GTACGAG33400.016.88240419
Produced by FastQC (version 0.11.2)