FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21106474
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT218080.10332374796472399Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT22800.059.96686694
CGACGTA33450.047.78833492
TGTCGGT28450.044.471493
ACGCCGT27600.044.3622594
TCGACGT36900.043.32055391
ACGTATC35400.042.46578694
TACGAGT17850.041.1562494
TGATCGC25900.039.6576892
GTGATCG25800.039.54795591
TAGATCG25950.039.32073688
GGTCGAC42850.037.46446689
GTGGACG36050.037.17188690
GTCGGTG35300.036.03437894
TGGTCGA45700.035.1288288
ATCGCCG27450.034.94032794
GTCGATC31450.034.60584392
CACCGTA40100.034.4351493
GTCGACG47200.034.01133390
GTGTCGA32400.033.80138890
CGGTGAT31300.033.46838489
Produced by FastQC (version 0.11.2)