FastQCFastQC Report
Mon 11 Dec 2017
AJ-F-ATAC-2-1_TAAGGCGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAJ-F-ATAC-2-1_TAAGGCGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21106474
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32400.033.1453821
TTGACGG37750.027.6505731
TACGGTG27450.024.03863314
CTACGGT27500.023.34096113
TGACGGC46650.022.76110632
GACGGCT45000.022.52910433
TCCGCAA30450.020.48876433
CTAGACG33950.019.9685483
TAGGACG64100.019.0937064
CCTACGG33800.018.99042712
CGGCTAT61000.018.68550535
CATCGTT36150.018.41986732
GGTACGA32650.018.37388218
CCGTACG33800.018.1031959
TGCGACC36150.018.08797610
GTCCGCA33850.018.07645632
AACTGCG42250.017.8903457
CGTACGT32250.017.85719710
TAGACGC67050.017.8062484
GTACGAG34450.017.58786819
Produced by FastQC (version 0.11.2)