FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16733428
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT441340.2637475118666659Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21000.025.4332681
TAGGACG60850.020.2173734
CGGTGGG70900.019.7179120
TCTCGGG48200.018.547707120
AGACCGT31550.017.8754146
TTGACGG27750.017.51423631
CGCCGTT31600.017.278471120
CGTATCC143950.017.13094120
GGACGTG72550.017.03566
GACCGTT27150.016.7945657
TCGCCGG92500.016.28107120
GTCCTAC96850.016.1103571
AGGACGT91550.015.0095745
AAGACCG37100.014.8800755
ACCGTTT28650.014.8680798
CTACAGT111250.014.3487184
GACGAGG89750.014.239546120
GTAGGAC98000.014.2067063
TCAACGG23650.014.20620313
GTATACG6357.1486284E-1014.1758461
Produced by FastQC (version 0.11.2)