FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16733428
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA416860.24911811255888514No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT221660.13246538605239763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21800.029.4438171
TAGGACG57100.020.383464
TTGACGG28250.020.17180431
GTCCTAC92650.017.8054981
TTAGACG21600.017.7761823
ATACGCG27550.017.639074
ACGCGTA27250.017.172316
TAGACGC38550.017.1190854
TACGCGT27350.017.109935
GTAGGAC96800.016.8579923
GCGTAAA20300.016.5485081
AGACCGT31650.015.9223126
AACTGCG31050.015.8427577
TACAGTG125400.015.7401675
CGGCTAT37400.015.71802535
CGCGTAT30450.015.3668847
GGACGTG73100.015.3470476
ACGGCTA36150.015.26592234
ACTGCGA39950.015.1664218
TGCACCG26350.015.0270695
Produced by FastQC (version 0.11.2)