FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16730258
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT441540.2639170298509443Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG47100.023.057287120
CGTCTAG22550.021.288861
CGGTGGG71950.019.51352120
TAGGACG59800.019.4674724
CGTATCC148050.019.128643120
GTCCTAC97950.017.5214711
CGCCGTT30550.016.693918120
CCCGATA33650.016.2250048
TCGCCGG93350.016.00426120
TTGACGG27900.015.48528131
TGTTCCG41200.015.4372845
GGACGTG72050.015.4052526
AACTGCG30800.015.38880357
CCGATAC25200.014.7611319
TGTACCG11450.014.672865
TACAGTG125200.014.6169835
AGACCGT30150.014.5266896
GTAGGAC102900.014.46256353
CGTCTAA22500.014.4019141
CCGACGG48000.014.374978120
Produced by FastQC (version 0.11.2)