FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16730258
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA418530.2501635061455717No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT219590.13125320601750431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18850.029.2780531
TAGGACG59200.021.5857834
CTACGGT19800.020.60083413
CTAGACG21750.019.5843413
GGACGTG67800.018.669956
GTCCTAC100550.018.1366581
TACGGTG21800.017.3350514
TGCGACC26350.017.30111310
TTGACGG26800.017.23474331
AGACCGT28700.016.9314276
AACTGCG32000.016.8724337
CCTACGG25250.016.15431812
AGGACGT91850.016.1331775
TACGCCG21050.015.9601895
ACTGCGA41500.015.90119658
CTACACG27250.015.8517024
TACACGG24950.015.6294945
TAGACGC40550.015.5348944
GTACCGT8900.015.5034476
CGACCAA28800.015.20447512
Produced by FastQC (version 0.11.2)