FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16674386
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT436600.26183872677530673Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG49600.022.136885120
CGGTGGG68300.020.64402120
CGTCTAG21200.018.3986971
TAGGACG57750.018.3920824
CGTATCC150850.018.216597120
GTCCTAC97050.017.0656621
CGCCGTT30650.016.247805120
TCGCCGG86700.015.709191120
CCGACGG46500.015.483723120
GGACGTG69100.015.1085296
TACGCGT29150.015.0264415
ATACGCG29650.014.9767174
CTACAGT111200.014.8401234
TACAGTG125000.014.7846995
CGTACGT19900.014.77656434
TTGGACG25250.014.73178117
TTGACGG25650.014.50687331
GACGAGG86500.014.496971120
CGTAATC24450.014.47873522
AACTGCG30300.014.2572037
Produced by FastQC (version 0.11.2)