FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16674386
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA413710.24811108486993164No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT219590.13169300506777282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG19500.027.6876661
TAGGACG60100.021.2632274
TTGACGG25700.018.67240131
TACGGTG18650.018.01117714
GGACGTG71400.017.5599256
CTACGGT20000.016.49550413
GTAGGAC101700.016.2231733
GTCCTAC93700.016.0058561
CTAGACG22650.015.8930443
CGGCTAT34700.015.55832635
TACGCGT29000.015.5162715
GCCTAAC43450.015.4634611
ATACGCG29950.015.4247444
CGTACGT18100.015.24505834
TAGACGC36800.015.1620944
ACGCGTA29700.015.1488416
AGGACGT92350.015.0721475
CCGTACG18850.014.95649533
CCCGATA32900.014.9518138
GTATAGG58000.014.7906261
Produced by FastQC (version 0.11.2)