FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16666383
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT437060.2622404633326859Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG51700.021.700832120
CGTCTAG20900.019.8113541
TAGGACG59700.019.8010794
CGTATCC140750.018.585035120
CGCCGTT34550.018.407005120
CGGTGGG69650.018.17551120
TCGCCGG86850.018.030012120
GTCCTAC97750.016.4524041
TACGCGT27050.015.5284395
ATACGCG27100.015.2783614
AGACCGT28550.015.1326696
ACGCGTA28100.014.9479256
CTACAGT109800.014.8649534
TACAGTG126150.014.8410415
CCCGATA32950.014.7489188
CGCGTAT27000.014.6673327
GGACGTG70450.014.6499676
CTAGACG21100.014.5043753
CTGGTCG41450.014.4744079
CTACGGT16700.014.37078813
Produced by FastQC (version 0.11.2)