FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16666383
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA414460.24868023253755778No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT214100.12846218642641297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20000.028.7970261
TAGGACG60950.020.86814
CTACGGT19100.020.41410613
CTAGACG21700.019.629963
TACGGTG20750.018.79115514
TTGACGG26600.018.49133531
CGTACGT18750.017.9162710
GTCCTAC98500.017.5413361
CCTACGG23150.016.84274112
GGACGTG72850.016.387566
CCGTACG20150.016.0763459
TAGACGC38450.016.0716934
GTTCCGT39650.015.7355146
TACAGTG129250.015.5975195
GTAGGAC99900.015.4343523
AGGACGT93500.015.3367425
AAGACCG33300.015.315165
CTACACG26650.015.3084944
TGTTCCG40450.014.9813055
CGGCTAT35600.014.82752735
Produced by FastQC (version 0.11.2)