FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16618193
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT436280.2625315520165159Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG23100.025.7195011
TCTCGGG50750.023.643215120
CGGTGGG71050.020.941133120
TAGGACG57200.018.8846654
TTGACGG26750.018.84545531
CGTATCC153700.018.579998120
GTCCTAC97250.017.6488251
TCGCCGG91200.017.366875120
GGACGTG67900.015.9077256
TACAGTG130000.015.602935
TGTACCG13100.015.5754445
TGACGCC65400.015.41147120
AACTGCG32000.015.3755287
ACTGCGA37500.015.3599268
CGAATTG24700.014.81854622
TAGACCG21900.014.7972994
GGTACGA18800.014.68082518
CGGCTAT36550.014.61247235
CTACAGT111700.014.3984074
TGCGACC25800.014.18491210
Produced by FastQC (version 0.11.2)