FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16618193
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA415470.2500091315584071No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT216700.1303992558035642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20350.028.3022021
TAGGACG63800.022.2861234
GGACGTG74700.017.7490316
GTCCTAC93650.017.5531941
AACTGCG32100.017.3800417
GTAGGAC101550.017.310163
TTGACGG29850.017.28222331
TACGCGT29450.016.9082895
CTACGGT21400.016.2577213
ATACGCG29700.016.1599664
TACGGTG19400.016.08024814
CTAGACG23150.016.0677133
AGGACGT96050.016.0524985
ACGCGTA29750.015.9316
CGTACGT19200.015.30973610
GTATAGG52950.015.2961171
TACACGG25350.015.146385
TGCGACC27650.014.97017310
TACACCG26100.014.9410015
CGTCTAA21350.014.8933151
Produced by FastQC (version 0.11.2)