FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16636463
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT429250.25801758462721314Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21750.023.1794511
TCTCGGG52800.021.70187120
TAGGACG58100.019.420294
CGTATCC159150.017.679255120
CGGTGGG68250.017.140745120
AGACCGT30250.016.6639616
CGTACGT20750.016.48049210
GGTACGA19950.015.94061718
TTGACGG27550.015.90161631
CGCCGTT31700.015.897094120
CCGTACG23150.015.8080349
GGTCGGT40950.015.23717911
GGACGTG70350.015.1837916
CGGCTAT35100.015.04553935
AACTGCG31550.014.8341787
GACCGTT25900.014.8268357
TACAGTG130750.014.7342565
ACCGTTT28150.014.7071048
TCGCCGG90650.014.625887120
GTACGAG21750.014.62165619
Produced by FastQC (version 0.11.2)