FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16636463
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA409820.24633841941042395No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT217590.13079102210608107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG19200.028.1202141
TAGGACG59150.022.3121474
CTACGGT19700.021.61945213
TACGGTG20500.020.48339714
TTGACGG26700.018.64666231
CTAGACG23550.018.5953943
GTCCTAC93050.018.2451481
TAGACGC39600.018.1785624
GGACGTG71650.017.8312596
CCTACGG25400.016.76769312
CGCATTA20100.016.11559512
TCCGCAA26700.015.95075833
AACTGCG33200.015.8987587
AGGACGT90600.015.8911935
CTACAGT111150.015.6517154
GTAGGAC102700.015.3623893
TACAGTG129400.015.2986515
TACACGG24900.014.9370835
GTCCGCA28700.014.83920932
AGAACCG33650.014.7967545
Produced by FastQC (version 0.11.2)